地膜覆盖下土壤有机质的分解与积累

还田有机物在地膜覆盖下的分解略高于露地栽培,腐殖化系数为0.17～0.41,土壤有机质的年亩矿化量为92～164kg.有机无机肥配施,对土壤有机质的贡献高于不施肥、单施化肥和单施有机肥,尤其是未经分解的有机物,适量配施化肥,明显优于等氮量的腐熟有机肥.为此,要重新组合农业措施.以调整土壤有机质分解与积累之间的关系.     

与大豆SMV3号株系抗性相关的分子标记的鉴定

对大豆花叶病毒SMV抗性的遗传研究一直是大豆抗病遗传研究的热点之一。本研究以哈91R3-301×黑农41组合构建了遗传群体,其F2分离单株的SSR标记基因型基本符合1:2:1的比例,说明这个群体没有偏分离。根据F3株系的病情指数分布推测SMV3的F2成株抗性似乎由多基因控制。根据SSR分子标记的基因型和F2:3株系对SMV3抗病性表型结果连锁分析,推测Satt296是与大豆花叶病毒(SMV)3号株系抗性主基因连锁的分子标记,应用Joinmap作图软件将该标记定位在D1b连锁群上,这一结果与部分文献报道的研究结果一致。本研究获得的与抗性基因连锁的分子标记在其他的RIL群体中的验证得到了初步证实,推测定位在D1b连锁群上的抗性座位可能是控制SMV3的主基因之一,该标记可望应用于大豆抗SMV3的分子标记辅助选择。     

低值水产品及水产副产品的加工与综合利用

低值水产品及其副产品通常经过简单加工后被作为饲料利用或直接作为废物丢弃,不仅造成资源浪费,而且造成对环境的污染。研究表明,利用高新技术,可以把低值水产品或水产品下脚料加工成功能性鱼油制品、新型鱼糜制品、海鲜调味品和营养丰富的水解鱼蛋白饲料等高附加值的产品,以及骨糊、硫酸软骨素和骨胶等功能性添加剂。这些产品的研究开发为低值水产品及副产品的加工利用提供了新的途径。     

A Simple and Reliable Assay for Detecting Specific Nucleotide Sequences in Plants Using Optical Thin-film Biosensor Chips

Here we report the adaptation and optimization of an efficient, accurate and inexpensive assay that employs custom-designed silicon-based optical thin-film biosensor chips to detect unique transgenes in genetically modified （GM） crops and SN-P markers in model plant genomes. Briefly, aldehyde-attached sequence-specific singlestranded oligonucleotide probes are arrayed and covalently attached to a hydrazine-derivatized biosensor chip surface. Unique DNA sequences （or genes） are detected by hybridizing biotinylated PCR amplicons of the DNA sequences to probes on the chip surface. In the SN-P assay, target sequences （PCR amplicons） are hybridized in the presence of a mixture of biotinylated detector probes and a thermostable DNA ligase. Only perfect matches between the probe and target sequences, but not those with even a single nucleotide mismatch, can be covalently fixed on the chip surface. In both cases, the presence of specific target sequences is siL, nified by a color change on the chip surface （gold to blue/purple） after brief incubation with an anti-biotin IgG horseradish peroxidase （HRP） to generate a precipitable product from an HRP substrate.     

Effect of high-pressure hot-water washing treatment on fruit quality, insects, and disease in apples and pears: Part I. System description and the effect on fruit quality of ‘d’Anjou’ pears

A manually operated high-pressure hot-water washing system consisting of a boiler, hot-water mixing tank, contact loop, heat exchanger, spray mixing tank, high-pressure hot-water washing manifold, low-pressure fresh water rinse manifold, and pressure pump was constructed and installed in a packingline. The system developed 20–50 °C washing water at pressures up to 980 kPa. ‘d’Anjou’ pears (Pyrus communis L.), shortly after harvest, and after storage for 3 and 4 months in regular air (RA) or for 4, 7 and 8 months in controlled atmosphere (CA) at −1 °C were washed through the packingline with different wetting agents (0.1% Silwet, 0.01 and 0.1% Defoamer, and water), water pressures (regular and high-pressure (210–980 kPa)), water temperatures (control (tap water, 4–22 °C), 40 °C, and 50 °C), and brushes (soft and firm), respectively. The effect of the washing conditions on fruit quality was investigated after 1 month of storage at −1 °C to simulate shipping condition, and then again after 1 week at 20 °C to simulate marketing condition. Hot-water caused severe heat scald. When nozzle temperature was 50 °C, the incidence of heat scald increased to over 50% for the fruit stored in RA for 3 months. Combined with hot-water, 540 kPa high-pressure washing increased the incidence of friction discoloration. There were lower incidences of friction discoloration and heat scald for fruit stored in CA for 7 months, in comparison to that in RA for 3 months. However, those fruit did not ripen properly as indicated by a high extractable juice content. Fruit washed at harvest had minor incidences of friction discoloration regardless of different brushes, water pressures, and wetting agents. Fruit washed after storages in either 4 months RA or 4 or 8 months CA suffered a high incidence of friction discoloration including scuffing symptoms and pressure marking. The firm brushes caused a higher incidence of friction discoloration mainly because of scuffing symptoms. However, no differences were found between different water pressures and wetting agents with respect to friction discoloration. Fruit stored for 4 months RA suffered 26–28% friction discoloration in comparison to 16–18% in CA stored fruit with firm brush washing. Extended CA storage increased friction discoloration even with soft brush washing. The results suggest that a washing system with high-pressure spray, <30 °C warm water, wetting agent Defoamer and rotating soft brushes were significantly effective in removing surface pests and decay control without causing internal or external damage of fruit.     

Factors influencing tissue culture responses of mature seeds and immature embryos in turf-type tall fescue

In an effort to optimize tissue culture responses of turf‐type tall fescue for genetic transformation, the effects of five culture medium supplements on tissue culture responses were investigated with immature embryos and mature seeds of an elite cultivar, ‘Coronado’, as explant tissues. For both explants, calli induced on 6‐benzylaminopurine (BAP)‐containing medium had significantly improved regeneration ability. The optimal concentration of BAP for the induction of regenerable callus from mature seeds was 0.1 mg/l. Thidiazuron also improved callus regeneration frequency of mature seeds. Casein hydrolysate, L‐proline and myo‐inositol improved callus induction of immature embryos but not mature seeds. They did not improve callus regeneration frequencies with either explant tissue. By simply slicing the mature seeds into two halves longitudinally, the callus induction frequencies, as well as the corresponding overall plant regeneration frequencies, were increased approximately three‐ to six‐fold in all three combinations of 2,4‐dichlorophenoxyacetic acid and BAP in callus induction media.     

高粱非买罗细胞质A_2、A_3雄性不育系研究

非买罗细胞质A_2、A_3雄性不育系的研究表明:现有A_1系统保持材料对A_2、A_3仍具有保持能力,A_2、A_3系统的保持系对A_1表现为恢复.A_1系统恢复系,不同生态型的材料对A_2恢复能力不同,中国高粱仍具恢复能力,部分印度高粱表现为保持,A_1、A_2的恢复系对A_3均表现为保持.研究认为:A_2扩大了细胞质利用范围,加强“A_2带”的利用,可有效地促进高粱育种的生产.     

柴油机喷嘴内空穴流动可视化试验与数值模拟

柴油机喷雾特性除了与气缸内气流运动有关外,也与喷嘴内空穴流动有密切的关系.设计了比例放大多孔喷嘴内空穴流动和喷雾的可视化试验装置,进行了不同燃油喷射压力下喷嘴内空穴现象和喷雾的可视化试验,对所建喷嘴内空穴流动的三维数值计算模型进行了试验验证.采用试验和数值模拟相结合的方法分析了喷嘴内部流动从单相湍流流动发展为空穴流动、超空穴流动的流动特性,得出了喷嘴流量系数与喷射压力及空穴参数之间的关系以及喷嘴内出现空穴现象的临界条件.     

野生动物旅游产品现状初探

野生动物旅游产品在总体的旅游产品中占有很大的市场份额,也有越来越多的旅游爱好者对此拥有购买动机与欲望。根据目前旅游市场现状,本文对野生动物旅游产品的种类进行了归类,提出了野生动物旅游产品可分为观赏型、参与型、工艺品型、食用型和药用型等5种表现形式。结合中国在野生动物旅游产品方面的开发现状,从生态保护、增强体验性与真实性、社区共管、科普教育、法制建设和科学投资方面提出了合理开发的建议。     

精氨酸的抗热应激作用及在母猪上的应用

精氨酸是一种条件性必需氨基酸,在生理调节方面具有重要的功能。在正常情况下,成年动物对精氨酸的需求可以通过内源性合成满足,但在应激条件下,内源合成的精氨酸不足以满足机体的需求。精氨酸代谢可合成一氧化氮(NO),NO在体内发挥着重要的生理作用。文中综述了高温热应激对母猪的影响、精氨酸在体内的代谢过程及在抗氧化应激方面的作用,以促进高温热应激条件下精氨酸在母猪中的应用及研究。